Review



human tnbc cell lines mda mb 231  (ATCC)


Bioz Verified Symbol ATCC is a verified supplier
Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 99
      Buy from Supplier

    Structured Review

    ATCC human tnbc cell lines mda mb 231
    Human Tnbc Cell Lines Mda Mb 231, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 24645 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mda+mb+231+tnbc/MDA-MB-231/pmc13266114-183-1-6
    Average 99 stars, based on 24645 article reviews
    human tnbc cell lines mda mb 231 - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    Multiple Displacement Amplification:

    Article Title: Single amino-acid differences define H2B variants and modify chromatin accessibility to induce EMT in breast cancer.
    Article Snippet: This cell line was cultured as previously described [41] in phenol red-free DMEM/F12 media supplemented with 5% horse serum (Gibco), 1X penicillin-streptomycin (Gibco), 20 ng/mL EGF (Peprotech), 0.5 μg/mL hydrocortisone (Sigma-Aldrich), 100 ng/mL cholera toxin (Sigma-Aldrich), and 10 μg/mL insulin (Sigma-Aldrich). .. The human breast cancer cell lines MCF7 (metastatic luminal A), T47D (luminal A), BT474 (luminal B), and MDA-MB-231 (TNBC) were obtained from ATCC. .. MDA-MB-231 was cultured in phenol red-free, high glucose DMEM (Gibco) supplemented with 10% FBS (Gibco).

    Article Title: Single amino-acid differences define H2B variants and modify chromatin accessibility to induce EMT in breast cancer
    Article Snippet: This cell line was cultured as previously described [ ] in phenol red-free DMEM/F12 media supplemented with 5% horse serum (Gibco), 1X penicillin-streptomycin (Gibco), 20 ng/mL EGF (Peprotech), 0.5 μg/mL hydrocortisone (Sigma-Aldrich), 100 ng/mL cholera toxin (Sigma-Aldrich), and 10 μg/mL insulin (Sigma-Aldrich). .. The human breast cancer cell lines MCF7 (metastatic luminal A), T47D (luminal A), BT474 (luminal B), and MDA-MB-231 (TNBC) were obtained from ATCC. .. MDA-MB-231 was cultured in phenol red-free, high glucose DMEM (Gibco) supplemented with 10% FBS (Gibco).

    Article Title: Incorporating histone H2B variants into chromatin modifies chromatin accessibility to induce epithelial-to-mesenchymal transition in breast cancer
    Article Snippet: This cell line was cultured as previously described [ ] in phenol red-free DMEM/F12 media supplemented with 5% horse serum (Gibco), 1X penicillin-streptomycin (Gibco), 20 ng/mL EGF (Peprotech), 0.5 μg/mL hydrocortisone (Sigma-Aldrich), 100 ng/mL cholera toxin (Sigma-Aldrich), and 10 μg/mL insulin (Sigma-Aldrich). .. The human breast cancer cell lines MCF7 (metastatic luminal A), T47D (luminal A), BT474 (luminal B), and MDA-MB-231 (TNBC) were obtained from ATCC. .. MDA-MB-231 was cultured in phenol red-free, high glucose DMEM (Gibco) supplemented with 10% FBS (Gibco).

    Article Title: EDIL3/Del-1-Dependent Induction of AMPKβ Phosphorylation Regulates the Progression of Mesenchymal Stem-like Triple-Negative Breast Cancer
    Article Snippet: The following reagents and antibodies against the following proteins were used: A769662 (3336; Tocris, Bristol, United Kingdom); AMPKβ1/2 (4150), total AMPK (2532), and phosphorylated AMPKβ (p-AMPKβ; S108) (23021) from Cell Signaling Technology (Danvers, MA, USA); GAPDH (sc-47724) from Santa Cruz Biotechnology (Dallas, TX, USA); Del-1 (PA5-27994) from Thermo Fisher Scientific (Rockford, IL, USA); and HA (ab236632) from Abcam (Waltham, MA, USA). .. Breast cancer cell lines used included MCF10A (normal breast cell), MCF-7 (luminal A), BT-474 (luminal B), SK-BR-3 (HER2), MDA-MB-231 (TNBC), and Hs 578T (TNBC), all obtained from ATCC. .. Cells were cultured in a humidified incubator at 37 °C with 5% CO 2 in Dulbecco’s Modified Eagle Medium (DMEM, high glucose; SH30243.01; Cytiva, Logan, UT, USA), supplemented with 10% fetal bovine serum (FBS; 16000044; Gibco, Grand Island, NE, USA).

    Article Title: EphB2-Targeting Monoclonal Antibodies Exerted Antitumor Activities in Triple-Negative Breast Cancer and Lung Mesothelioma Xenograft Models.
    Article Snippet: .. CHO-K1, MDA-MB-231 (TNBC), NCI-H226 (lung mesothelioma), and LS174T (colorectal cancer) cell lines were obtained from the American Type Culture Collection (Manassas, VA, USA). ..

    Article Title: EDIL3/Del-1-Dependent Induction of AMPKβ Phosphorylation Regulates the Progression of Mesenchymal Stem-like Triple-Negative Breast Cancer.
    Article Snippet: The following reagents and antibodies against the following proteins were used: A769662 (3336; Tocris, Bristol, United Kingdom); AMPKβ1/2 (4150), total AMPK (2532), and phosphorylated AMPKβ (p-AMPKβ; S108) (23021) from Cell Signaling Technology (Danvers, MA, USA); GAPDH (sc-47724) from Santa Cruz Biotechnology (Dallas, TX, USA); Del-1 (PA5-27994) from Thermo Fisher Scientific (Rockford, IL, USA); and HA (ab236632) from Abcam (Waltham, MA, USA). .. Breast cancer cell lines used included MCF10A (normal breast cell), MCF-7 (luminal A), BT-474 (luminal B), SK-BR-3 (HER2), MDA-MB-231 (TNBC), and Hs 578T (TNBC), all obtained from ATCC. .. Cells were cultured in a humidified incubator at 37 ◦C with 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM, high glucose; SH30243.01; Cytiva, Logan, UT, USA), supplemented with 10% fetal bovine serum (FBS; 16000044; Gibco, Grand Island, NE, USA).

    Article Title: CLIC3 is upregulated across all subtypes of breast cancer and plays a key role in cell migration, invasion and growth in soft agar.
    Article Snippet: .. Cell Culture, Transfection and shRNA Knockdown The human breast cancer cell lines, T47D (Luminal A; ER+), HCC1954 (HER2+), HCC1806 (TNBC), MDA-MB-231 (TNBC) and the human non-tumorigenic breast epithelial cell line, MCF10A, were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). .. Cells were maintained according to ATCC recommendations for up to six months from the time of resuscitation.

    Article Title: EphB2-Targeting Monoclonal Antibodies Exerted Antitumor Activities in Triple-Negative Breast Cancer and Lung Mesothelioma Xenograft Models
    Article Snippet: .. CHO-K1, MDA-MB-231 (TNBC), NCI-H226 (lung mesothelioma), and LS174T (colorectal cancer) cell lines were obtained from the American Type Culture Collection (Manassas, VA, USA). ..

    Cell Culture:

    Article Title: CLIC3 is upregulated across all subtypes of breast cancer and plays a key role in cell migration, invasion and growth in soft agar.
    Article Snippet: .. Cell Culture, Transfection and shRNA Knockdown The human breast cancer cell lines, T47D (Luminal A; ER+), HCC1954 (HER2+), HCC1806 (TNBC), MDA-MB-231 (TNBC) and the human non-tumorigenic breast epithelial cell line, MCF10A, were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). .. Cells were maintained according to ATCC recommendations for up to six months from the time of resuscitation.

    Transfection:

    Article Title: CLIC3 is upregulated across all subtypes of breast cancer and plays a key role in cell migration, invasion and growth in soft agar.
    Article Snippet: .. Cell Culture, Transfection and shRNA Knockdown The human breast cancer cell lines, T47D (Luminal A; ER+), HCC1954 (HER2+), HCC1806 (TNBC), MDA-MB-231 (TNBC) and the human non-tumorigenic breast epithelial cell line, MCF10A, were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). .. Cells were maintained according to ATCC recommendations for up to six months from the time of resuscitation.

    shRNA:

    Article Title: CLIC3 is upregulated across all subtypes of breast cancer and plays a key role in cell migration, invasion and growth in soft agar.
    Article Snippet: .. Cell Culture, Transfection and shRNA Knockdown The human breast cancer cell lines, T47D (Luminal A; ER+), HCC1954 (HER2+), HCC1806 (TNBC), MDA-MB-231 (TNBC) and the human non-tumorigenic breast epithelial cell line, MCF10A, were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). .. Cells were maintained according to ATCC recommendations for up to six months from the time of resuscitation.

    Knockdown:

    Article Title: CLIC3 is upregulated across all subtypes of breast cancer and plays a key role in cell migration, invasion and growth in soft agar.
    Article Snippet: .. Cell Culture, Transfection and shRNA Knockdown The human breast cancer cell lines, T47D (Luminal A; ER+), HCC1954 (HER2+), HCC1806 (TNBC), MDA-MB-231 (TNBC) and the human non-tumorigenic breast epithelial cell line, MCF10A, were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). .. Cells were maintained according to ATCC recommendations for up to six months from the time of resuscitation.



    Similar Products

    human tnbc cell lines mda mb 231  (ATCC)
    99
    ATCC human tnbc cell lines mda mb 231
    Human Tnbc Cell Lines Mda Mb 231, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mda+mb+231+tnbc/MDA-MB-231/pmc13266114-183-1-6
    Average 99 stars, based on 1 article reviews
    human tnbc cell lines mda mb 231 - by Bioz Stars, 2026-10
    99/100 stars
    		  Buy from Supplier
    tnbc cell lines mda mb 231  (Procell Inc)
    86
    Procell Inc tnbc cell lines mda mb 231
    CEP exerts cytotoxicity in TNBC cells. (A) Chemical structure of CEP. (B) CEP inhibited TNBC cell viability. The effect of CEP treatment on TNBC cell viability after 24, 48 and 72 h. (C) CEP inhibited <t>clonogenicity</t> <t>of</t> <t>MDA-MB-231</t> and Hs578T cells. n=3; *P < 0.05, **P < 0.01 and ***P < 0.001 vs. control. CEP, cepharanthine; TNBC, triple-negative breast cancer.
    Tnbc Cell Lines Mda Mb 231, supplied by Procell Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mda+mb+231+tnbc/231+mb+mda/pmc13169123-22-1-8
    Average 86 stars, based on 1 article reviews
    tnbc cell lines mda mb 231 - by Bioz Stars, 2026-10
    86/100 stars
    		  Buy from Supplier
    mda mb 231 tnbc  (Procell Inc)
    86
    Procell Inc mda mb 231 tnbc
    CEP exerts cytotoxicity in TNBC cells. (A) Chemical structure of CEP. (B) CEP inhibited TNBC cell viability. The effect of CEP treatment on TNBC cell viability after 24, 48 and 72 h. (C) CEP inhibited <t>clonogenicity</t> <t>of</t> <t>MDA-MB-231</t> and Hs578T cells. n=3; *P < 0.05, **P < 0.01 and ***P < 0.001 vs. control. CEP, cepharanthine; TNBC, triple-negative breast cancer.
    Mda Mb 231 Tnbc, supplied by Procell Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mda+mb+231+tnbc/231+mb+mda/pm42308796-63-0-17
    Average 86 stars, based on 1 article reviews
    mda mb 231 tnbc - by Bioz Stars, 2026-10
    86/100 stars
    		  Buy from Supplier
    tnbc cell line mda mb 231  (Procell Inc)
    86
    Procell Inc tnbc cell line mda mb 231
    CEP exerts cytotoxicity in TNBC cells. (A) Chemical structure of CEP. (B) CEP inhibited TNBC cell viability. The effect of CEP treatment on TNBC cell viability after 24, 48 and 72 h. (C) CEP inhibited <t>clonogenicity</t> <t>of</t> <t>MDA-MB-231</t> and Hs578T cells. n=3; *P < 0.05, **P < 0.01 and ***P < 0.001 vs. control. CEP, cepharanthine; TNBC, triple-negative breast cancer.
    Tnbc Cell Line Mda Mb 231, supplied by Procell Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mda+mb+231+tnbc/231+mb+mda/pm42310796-114-1-17
    Average 86 stars, based on 1 article reviews
    tnbc cell line mda mb 231 - by Bioz Stars, 2026-10
    86/100 stars
    		  Buy from Supplier
    human mesenchymal tnbc mda mb 231 cells  (ATCC)
    99
    ATCC human mesenchymal tnbc mda mb 231 cells
    CEP exerts cytotoxicity in TNBC cells. (A) Chemical structure of CEP. (B) CEP inhibited TNBC cell viability. The effect of CEP treatment on TNBC cell viability after 24, 48 and 72 h. (C) CEP inhibited <t>clonogenicity</t> <t>of</t> <t>MDA-MB-231</t> and Hs578T cells. n=3; *P < 0.05, **P < 0.01 and ***P < 0.001 vs. control. CEP, cepharanthine; TNBC, triple-negative breast cancer.
    Human Mesenchymal Tnbc Mda Mb 231 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mda+mb+231+tnbc/MDA-MB-231/pm42308957-61-0-5
    Average 99 stars, based on 1 article reviews
    human mesenchymal tnbc mda mb 231 cells - by Bioz Stars, 2026-10
    99/100 stars
    		  Buy from Supplier
    human triple negative breast cancer tnbc mda mb 231 cells  (ATCC)
    99
    ATCC human triple negative breast cancer tnbc mda mb 231 cells
    CEP exerts cytotoxicity in TNBC cells. (A) Chemical structure of CEP. (B) CEP inhibited TNBC cell viability. The effect of CEP treatment on TNBC cell viability after 24, 48 and 72 h. (C) CEP inhibited <t>clonogenicity</t> <t>of</t> <t>MDA-MB-231</t> and Hs578T cells. n=3; *P < 0.05, **P < 0.01 and ***P < 0.001 vs. control. CEP, cepharanthine; TNBC, triple-negative breast cancer.
    Human Triple Negative Breast Cancer Tnbc Mda Mb 231 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mda+mb+231+tnbc/MDA-MB-231/pm42252443-132-0-8
    Average 99 stars, based on 1 article reviews
    human triple negative breast cancer tnbc mda mb 231 cells - by Bioz Stars, 2026-10
    99/100 stars
    		  Buy from Supplier
    tnbc cell culture human tnbc mda mb 231 cells  (ATCC)
    99
    ATCC tnbc cell culture human tnbc mda mb 231 cells
    CEP exerts cytotoxicity in TNBC cells. (A) Chemical structure of CEP. (B) CEP inhibited TNBC cell viability. The effect of CEP treatment on TNBC cell viability after 24, 48 and 72 h. (C) CEP inhibited <t>clonogenicity</t> <t>of</t> <t>MDA-MB-231</t> and Hs578T cells. n=3; *P < 0.05, **P < 0.01 and ***P < 0.001 vs. control. CEP, cepharanthine; TNBC, triple-negative breast cancer.
    Tnbc Cell Culture Human Tnbc Mda Mb 231 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mda+mb+231+tnbc/MDA-MB-231/pm42243938-85-0-8
    Average 99 stars, based on 1 article reviews
    tnbc cell culture human tnbc mda mb 231 cells - by Bioz Stars, 2026-10
    99/100 stars
    		  Buy from Supplier
    human tnbc mda mb 231 cells  (ATCC)
    99
    ATCC human tnbc mda mb 231 cells
    CEP exerts cytotoxicity in TNBC cells. (A) Chemical structure of CEP. (B) CEP inhibited TNBC cell viability. The effect of CEP treatment on TNBC cell viability after 24, 48 and 72 h. (C) CEP inhibited <t>clonogenicity</t> <t>of</t> <t>MDA-MB-231</t> and Hs578T cells. n=3; *P < 0.05, **P < 0.01 and ***P < 0.001 vs. control. CEP, cepharanthine; TNBC, triple-negative breast cancer.
    Human Tnbc Mda Mb 231 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mda+mb+231+tnbc/MDA-MB-231/pm42230497-45-0-8
    Average 99 stars, based on 1 article reviews
    human tnbc mda mb 231 cells - by Bioz Stars, 2026-10
    99/100 stars
    		  Buy from Supplier
    triple negative breast cancer tnbc line mda mb 231  (ATCC)
    99
    ATCC triple negative breast cancer tnbc line mda mb 231
    a. Cell <t>viability</t> <t>of</t> <t>MDA-MB-231</t> cells treated in the absence (control) or presence of 20 µM NPs, 1µM PTX, and a combination of NPs with PTX. b . Combination index of synergy between PTX and NPs assessed by Chou–Talalay analysis. c . Dose reduction index analysis of PTX and NP combinations assessed by Chou–Talalay analysis. d . Calcein/PI staining of cells treated with 1 µM PTX and/or 20 µM NPs. Data are expressed as mean ± SD (n = 3). Scale bar shows 20μm.
    Triple Negative Breast Cancer Tnbc Line Mda Mb 231, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mda+mb+231+tnbc/RAW+264%2E7/bio_rxiv__64898__2026__05__28__728138-74-2-11
    Average 99 stars, based on 1 article reviews
    triple negative breast cancer tnbc line mda mb 231 - by Bioz Stars, 2026-10
    99/100 stars
    		  Buy from Supplier
    culture conditions human tnbc cells lines mda mb 231  (Procell Inc)
    86
    Procell Inc culture conditions human tnbc cells lines mda mb 231
    a. Cell <t>viability</t> <t>of</t> <t>MDA-MB-231</t> cells treated in the absence (control) or presence of 20 µM NPs, 1µM PTX, and a combination of NPs with PTX. b . Combination index of synergy between PTX and NPs assessed by Chou–Talalay analysis. c . Dose reduction index analysis of PTX and NP combinations assessed by Chou–Talalay analysis. d . Calcein/PI staining of cells treated with 1 µM PTX and/or 20 µM NPs. Data are expressed as mean ± SD (n = 3). Scale bar shows 20μm.
    Culture Conditions Human Tnbc Cells Lines Mda Mb 231, supplied by Procell Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mda+mb+231+tnbc/231+mb+mda/pm42159784-134-43-50
    Average 86 stars, based on 1 article reviews
    culture conditions human tnbc cells lines mda mb 231 - by Bioz Stars, 2026-10
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    CEP exerts cytotoxicity in TNBC cells. (A) Chemical structure of CEP. (B) CEP inhibited TNBC cell viability. The effect of CEP treatment on TNBC cell viability after 24, 48 and 72 h. (C) CEP inhibited clonogenicity of MDA-MB-231 and Hs578T cells. n=3; *P < 0.05, **P < 0.01 and ***P < 0.001 vs. control. CEP, cepharanthine; TNBC, triple-negative breast cancer.

    Journal: Molecular Medicine Reports

    Article Title: Cepharanthine inhibits lysosomes and induces apoptosis in triple-negative breast cancer cells

    doi: 10.3892/mmr.2026.13899

    Figure Lengend Snippet: CEP exerts cytotoxicity in TNBC cells. (A) Chemical structure of CEP. (B) CEP inhibited TNBC cell viability. The effect of CEP treatment on TNBC cell viability after 24, 48 and 72 h. (C) CEP inhibited clonogenicity of MDA-MB-231 and Hs578T cells. n=3; *P < 0.05, **P < 0.01 and ***P < 0.001 vs. control. CEP, cepharanthine; TNBC, triple-negative breast cancer.

    Article Snippet: The TNBC cell lines MDA-MB-231 (cat. no. CL-0150; Procell Life Science & Technology Co., Ltd.) and Hs578T (cat. no. CL-0114; Procell Life Science & Technology Co., Ltd.) were cultured in high-glucose DMEM medium (cat. no. C11965500BT; Gibco; Thermo Fisher Scientific, Inc.), supplemented with 10% fetal bovine serum (cat. no. 164210; Procell Life Science & Technology Co., Ltd.) and 1% penicillin/streptomycin (cat. no. 15140122; Gibco; Thermo Fisher Scientific, Inc.).

    Techniques: Control

    CEP induces apoptosis in triple-negative breast cancer cells. Flow cytometric quantification of apoptosis induced by CEP in (A) MDA-MB-231 and (B) Hs578T cells (n=3; 48 h). **P<0.01 and ***P < 0.001 vs. control. CEP, cepharanthine; ns, not significant.

    Journal: Molecular Medicine Reports

    Article Title: Cepharanthine inhibits lysosomes and induces apoptosis in triple-negative breast cancer cells

    doi: 10.3892/mmr.2026.13899

    Figure Lengend Snippet: CEP induces apoptosis in triple-negative breast cancer cells. Flow cytometric quantification of apoptosis induced by CEP in (A) MDA-MB-231 and (B) Hs578T cells (n=3; 48 h). **P<0.01 and ***P < 0.001 vs. control. CEP, cepharanthine; ns, not significant.

    Article Snippet: The TNBC cell lines MDA-MB-231 (cat. no. CL-0150; Procell Life Science & Technology Co., Ltd.) and Hs578T (cat. no. CL-0114; Procell Life Science & Technology Co., Ltd.) were cultured in high-glucose DMEM medium (cat. no. C11965500BT; Gibco; Thermo Fisher Scientific, Inc.), supplemented with 10% fetal bovine serum (cat. no. 164210; Procell Life Science & Technology Co., Ltd.) and 1% penicillin/streptomycin (cat. no. 15140122; Gibco; Thermo Fisher Scientific, Inc.).

    Techniques: Control

    CEP induces ΔΨm loss in triple-negative breast cancer cells. (A) MDA-MB-231 (10 µM; n=3; 24 h) and (B) Hs578T cells (4 µM; n=3; 24 h). Scale bar, 100 µM. CEP, cepharanthine.

    Journal: Molecular Medicine Reports

    Article Title: Cepharanthine inhibits lysosomes and induces apoptosis in triple-negative breast cancer cells

    doi: 10.3892/mmr.2026.13899

    Figure Lengend Snippet: CEP induces ΔΨm loss in triple-negative breast cancer cells. (A) MDA-MB-231 (10 µM; n=3; 24 h) and (B) Hs578T cells (4 µM; n=3; 24 h). Scale bar, 100 µM. CEP, cepharanthine.

    Article Snippet: The TNBC cell lines MDA-MB-231 (cat. no. CL-0150; Procell Life Science & Technology Co., Ltd.) and Hs578T (cat. no. CL-0114; Procell Life Science & Technology Co., Ltd.) were cultured in high-glucose DMEM medium (cat. no. C11965500BT; Gibco; Thermo Fisher Scientific, Inc.), supplemented with 10% fetal bovine serum (cat. no. 164210; Procell Life Science & Technology Co., Ltd.) and 1% penicillin/streptomycin (cat. no. 15140122; Gibco; Thermo Fisher Scientific, Inc.).

    Techniques:

    CEP upregulates NOXA and downregulates Bcl-2 expression in triple-negative breast cancer cells. CEP upregulated NOXA and downregulated Bcl-2 expression in (A) MDA-MB-231 cells (n=3; 24 h) and (B) Hs578T cells (n=3; 24 h). *P<0.05, **P<0.01 and ***P<0.001 vs. control. CEP, cepharanthine; NOXA, phorbol-12-myristate-13-acetate-induced protein 1; ns, not significant.

    Journal: Molecular Medicine Reports

    Article Title: Cepharanthine inhibits lysosomes and induces apoptosis in triple-negative breast cancer cells

    doi: 10.3892/mmr.2026.13899

    Figure Lengend Snippet: CEP upregulates NOXA and downregulates Bcl-2 expression in triple-negative breast cancer cells. CEP upregulated NOXA and downregulated Bcl-2 expression in (A) MDA-MB-231 cells (n=3; 24 h) and (B) Hs578T cells (n=3; 24 h). *P<0.05, **P<0.01 and ***P<0.001 vs. control. CEP, cepharanthine; NOXA, phorbol-12-myristate-13-acetate-induced protein 1; ns, not significant.

    Article Snippet: The TNBC cell lines MDA-MB-231 (cat. no. CL-0150; Procell Life Science & Technology Co., Ltd.) and Hs578T (cat. no. CL-0114; Procell Life Science & Technology Co., Ltd.) were cultured in high-glucose DMEM medium (cat. no. C11965500BT; Gibco; Thermo Fisher Scientific, Inc.), supplemented with 10% fetal bovine serum (cat. no. 164210; Procell Life Science & Technology Co., Ltd.) and 1% penicillin/streptomycin (cat. no. 15140122; Gibco; Thermo Fisher Scientific, Inc.).

    Techniques: Expressing, Control

    Proteomic profiling of MDA-MB-231 cells following CEP treatment. (A) The 20 most significantly downregulated Gene Ontology cellular components with CEP treatment. (B) Significantly downregulated Kyoto Encyclopedia of Genes and Genomes pathways with CEP treatment. CEP, cepharanthine.

    Journal: Molecular Medicine Reports

    Article Title: Cepharanthine inhibits lysosomes and induces apoptosis in triple-negative breast cancer cells

    doi: 10.3892/mmr.2026.13899

    Figure Lengend Snippet: Proteomic profiling of MDA-MB-231 cells following CEP treatment. (A) The 20 most significantly downregulated Gene Ontology cellular components with CEP treatment. (B) Significantly downregulated Kyoto Encyclopedia of Genes and Genomes pathways with CEP treatment. CEP, cepharanthine.

    Article Snippet: The TNBC cell lines MDA-MB-231 (cat. no. CL-0150; Procell Life Science & Technology Co., Ltd.) and Hs578T (cat. no. CL-0114; Procell Life Science & Technology Co., Ltd.) were cultured in high-glucose DMEM medium (cat. no. C11965500BT; Gibco; Thermo Fisher Scientific, Inc.), supplemented with 10% fetal bovine serum (cat. no. 164210; Procell Life Science & Technology Co., Ltd.) and 1% penicillin/streptomycin (cat. no. 15140122; Gibco; Thermo Fisher Scientific, Inc.).

    Techniques:

    CEP treatment activates TFEB in triple-negative breast cancer cells. (A) CEP triggers TFEB nuclear translocation in MDA-MB-231 cells (10 µM; 24 h). (B) CEP triggers TFEB nuclear translocation in Hs578T cells (4 µM; 24 h). Scale bar, 25 µm; n=5. **P<0.01 vs. control. TFEB, transcription factor EB; CEP, cepharanthine.

    Journal: Molecular Medicine Reports

    Article Title: Cepharanthine inhibits lysosomes and induces apoptosis in triple-negative breast cancer cells

    doi: 10.3892/mmr.2026.13899

    Figure Lengend Snippet: CEP treatment activates TFEB in triple-negative breast cancer cells. (A) CEP triggers TFEB nuclear translocation in MDA-MB-231 cells (10 µM; 24 h). (B) CEP triggers TFEB nuclear translocation in Hs578T cells (4 µM; 24 h). Scale bar, 25 µm; n=5. **P<0.01 vs. control. TFEB, transcription factor EB; CEP, cepharanthine.

    Article Snippet: The TNBC cell lines MDA-MB-231 (cat. no. CL-0150; Procell Life Science & Technology Co., Ltd.) and Hs578T (cat. no. CL-0114; Procell Life Science & Technology Co., Ltd.) were cultured in high-glucose DMEM medium (cat. no. C11965500BT; Gibco; Thermo Fisher Scientific, Inc.), supplemented with 10% fetal bovine serum (cat. no. 164210; Procell Life Science & Technology Co., Ltd.) and 1% penicillin/streptomycin (cat. no. 15140122; Gibco; Thermo Fisher Scientific, Inc.).

    Techniques: Translocation Assay, Control

    CEP does not induce LMP, increase lysosomal pH or destabilize lysosomal membrane-bound enzymes in triple-negative breast cancer cells. (A) CEP does not induce LMP, as evidenced by a dispersion of dextran, in MDA-MB-231 (10 µM; 24 h) and Hs578T cells (4 µM; 24 h). Scale bar, 25 µm. (B) CEP does not elevate lysosomal pH in the MDA-MB-231 (10 µM; 24 h) and Hs578T cell lines (4 µM; 24 h). Scale bar, 25 µm. (C) CEP does not induce lysosomal membrane-bound enzymes degradation in the MDA-MB-231 (10 µM; n=3; 24 h) and Hs578T cell lines (4 µM; n=3; 24 h). CEP, cepharanthine; ASAH1, acid ceramidase; SMPD1, sphingomyelin phosphodiesterase; ns, not significant.

    Journal: Molecular Medicine Reports

    Article Title: Cepharanthine inhibits lysosomes and induces apoptosis in triple-negative breast cancer cells

    doi: 10.3892/mmr.2026.13899

    Figure Lengend Snippet: CEP does not induce LMP, increase lysosomal pH or destabilize lysosomal membrane-bound enzymes in triple-negative breast cancer cells. (A) CEP does not induce LMP, as evidenced by a dispersion of dextran, in MDA-MB-231 (10 µM; 24 h) and Hs578T cells (4 µM; 24 h). Scale bar, 25 µm. (B) CEP does not elevate lysosomal pH in the MDA-MB-231 (10 µM; 24 h) and Hs578T cell lines (4 µM; 24 h). Scale bar, 25 µm. (C) CEP does not induce lysosomal membrane-bound enzymes degradation in the MDA-MB-231 (10 µM; n=3; 24 h) and Hs578T cell lines (4 µM; n=3; 24 h). CEP, cepharanthine; ASAH1, acid ceramidase; SMPD1, sphingomyelin phosphodiesterase; ns, not significant.

    Article Snippet: The TNBC cell lines MDA-MB-231 (cat. no. CL-0150; Procell Life Science & Technology Co., Ltd.) and Hs578T (cat. no. CL-0114; Procell Life Science & Technology Co., Ltd.) were cultured in high-glucose DMEM medium (cat. no. C11965500BT; Gibco; Thermo Fisher Scientific, Inc.), supplemented with 10% fetal bovine serum (cat. no. 164210; Procell Life Science & Technology Co., Ltd.) and 1% penicillin/streptomycin (cat. no. 15140122; Gibco; Thermo Fisher Scientific, Inc.).

    Techniques: Membrane, Dispersion

    CEP binds to and inhibits lysosomal enzymes. (A) Structurally altered peptides in the MDA-MB-231 cell line upon CEP treatment (10 µM, 1 h; FC >1.5 or <0.667, false discovery rate <1, P<0.01; n=3). (B) CEP treatment suppresses the maturation of CTSB and CTSD (24 h). *P<0.05, **P<0.01 and ***P<0.001 vs. control. CEP, cepharanthine; FC, fold change; CTSD, cathepsin D; CTSB, cathepsin B; pro-CTSB pro-cathepsin B; pro-CTSD, pro-cathepsin D; i-CTSB, inactive cathepsin B; m-CTSB, mature cathepsin B; m-CTSD, mature cathepsin D.

    Journal: Molecular Medicine Reports

    Article Title: Cepharanthine inhibits lysosomes and induces apoptosis in triple-negative breast cancer cells

    doi: 10.3892/mmr.2026.13899

    Figure Lengend Snippet: CEP binds to and inhibits lysosomal enzymes. (A) Structurally altered peptides in the MDA-MB-231 cell line upon CEP treatment (10 µM, 1 h; FC >1.5 or <0.667, false discovery rate <1, P<0.01; n=3). (B) CEP treatment suppresses the maturation of CTSB and CTSD (24 h). *P<0.05, **P<0.01 and ***P<0.001 vs. control. CEP, cepharanthine; FC, fold change; CTSD, cathepsin D; CTSB, cathepsin B; pro-CTSB pro-cathepsin B; pro-CTSD, pro-cathepsin D; i-CTSB, inactive cathepsin B; m-CTSB, mature cathepsin B; m-CTSD, mature cathepsin D.

    Article Snippet: The TNBC cell lines MDA-MB-231 (cat. no. CL-0150; Procell Life Science & Technology Co., Ltd.) and Hs578T (cat. no. CL-0114; Procell Life Science & Technology Co., Ltd.) were cultured in high-glucose DMEM medium (cat. no. C11965500BT; Gibco; Thermo Fisher Scientific, Inc.), supplemented with 10% fetal bovine serum (cat. no. 164210; Procell Life Science & Technology Co., Ltd.) and 1% penicillin/streptomycin (cat. no. 15140122; Gibco; Thermo Fisher Scientific, Inc.).

    Techniques: Control

    a. Cell viability of MDA-MB-231 cells treated in the absence (control) or presence of 20 µM NPs, 1µM PTX, and a combination of NPs with PTX. b . Combination index of synergy between PTX and NPs assessed by Chou–Talalay analysis. c . Dose reduction index analysis of PTX and NP combinations assessed by Chou–Talalay analysis. d . Calcein/PI staining of cells treated with 1 µM PTX and/or 20 µM NPs. Data are expressed as mean ± SD (n = 3). Scale bar shows 20μm.

    Journal: bioRxiv

    Article Title: Controlled delivery of iNOS antagonist, 1400W, for synergistic breast cancer therapy

    doi: 10.64898/2026.05.28.728138

    Figure Lengend Snippet: a. Cell viability of MDA-MB-231 cells treated in the absence (control) or presence of 20 µM NPs, 1µM PTX, and a combination of NPs with PTX. b . Combination index of synergy between PTX and NPs assessed by Chou–Talalay analysis. c . Dose reduction index analysis of PTX and NP combinations assessed by Chou–Talalay analysis. d . Calcein/PI staining of cells treated with 1 µM PTX and/or 20 µM NPs. Data are expressed as mean ± SD (n = 3). Scale bar shows 20μm.

    Article Snippet: The human triple-negative breast cancer (TNBC) line MDA-MB-231, murine macrophages RAW264.7 (ATCC), and human umbilical vein endothelial cells (HUVECs, ATCC, passages 6– 8) were from the American Type Culture Collection (ATCC) (Manassas, VA, USA).

    Techniques: Control, Staining